PRESENTACIÓN
Flagellin-PAc Fusion Protein Inhibits Progression of Established Caries.
Abstract
Dental caries remains one of the most common infectious diseases of humankind,
which develops slowly throughout life, affecting children, adolescents, and adults. A
vaccine against caries is urgently needed. We previously developed recombinant
flagellin as a mucosal adjuvant for anti-Streptococcus mutans vaccines by nasal
immunization. Furthermore, we demonstrated a fusion protein strategy that
combined flagellin and the target surface adhesion protein (PAc) in a single
construct. This construct enhanced specific IgA responses in oral fluids and provided
improved prophylactic protection against caries. In the present study, we observed
prolonged progression of dental caries in rats after S. mutans Ingbritt challenge. In
addition, we observed a therapeutic effect of the flagellin-PAc fusion protein (KFrPAc)
against dental caries as a mucosal vaccine with a new immunization protocol.
The present study demonstrated that KF-rPAc by nasal immunization can promote
PAc-specific systemic and mucosal antibody responses and inhibit dental caries
progression efficiently after the implant of S. mutans into the oral cavity of the rats.
The rats immunized with KF-rPAc exhibited 53.9% caries reduction compared with
the sham-immunized rats. Our data support the concept of administration of KF-rPAc
to humans after infection and even caries that has begun to alleviate caries
progression. In conclusion, our study demonstrated that KF-rPAc could be used as
an anticaries therapeutic mucosal vaccine.
sjimago:Q1
factor de impacto: 4.688
número de publicaciones del autor: 36
citaciones del artículo: 0
Año: 2015
revista: Journal dental research
Construction and Immunogenic Characterization of a Fusion Anti-caries DNA Vaccine against PAc and Glucosyltransferase I of Streptococcus mutans
Abstract
Glucosyltransferases (GTFs) and A cell-surface protein (PAc) are two important
virulence factors of the cariogenic organism Streptococcus mutans. They may
mediate sucrose-independent or sucrose-dependent attachment of Streptococcus
mutans to tooth surfaces, respectively. Thus, inhibiting both virulence factors is
predicted to provide better protection against caries than inhibiting a single factor.
To develop a highly efficient vaccineagainst caries, we constructed a fusion DNA
vaccine, pGLUA-P, by cloning the GLU region of GTF into a DNA vaccine, pCIA-P,
which encodes two highly conservative regions of PAc. In this report, we provide
evidence that fewer caries lesions were observed in rats following subcutaneous
injection of pGLUA-P, compared with pCIA-P, near the submandibular gland. Our
findings suggest that a multigenic DNA vaccine may be morecaries-preventive than
a single-gene DNA vaccine.
scjimago: Q1
Factor de impacto : 4.139
Numero de publicaciones del autor: 30
Citaciones del articulo:46
Revista: Journal dental research
The control of dental caries: can vaccines prevent cavities?
Abstract:
Essentially, responsibility for developing and implementing measures designed to
control dental caries should rest with the dental profession. Many of the most
effective ways of controlling caries, however, involve the use of fluoride. Either
systemically via fluoridated water and tablets or, topically, with fluoride-containing
toothpaste and mouthrinses. Scientists in many disciplines are aware that fluorides
can, in certain circumstances, endanger the environment. There is some evidence
that fluoride contamination of the total environment has increased in recent years.
The usefulness of fluoride as a caries preventive does not mean that unnecessary
exposure to the element should be tolerated. It is conceivable that fluoride is
approaching the limits of its effectiveness as a caries preventive. With this possibility
in mind, recent research directed toward the development of safe, effective anti-tooth
decay vaccines has been reviewed.
Revista: The Science of the total environment
Factor de impacto:4.099
Scimago:Q1.
Autor: Smith GE
publicaciones del autor:478.
Citaciones del artículo: 2
The scientific and public-health imperative for a vaccine against dental caries
Abstract
Dental caries is caused by one of the most ubiquitous bacterial infections of
humans. In many countries such as Brazil and China, this disease is reaching
epidemic proportions, and it is clear that a more effective public-health measure to
combat dental caries is needed, because disadvantaged children are the most
severely affected. One of the main groups of oral microorganisms, the mutans
streptococci, has been associated with the aetiology of dental caries, and
preclinical studies of immunological interventions have shown the feasibility of
interfering with this disease. Moreover, clinical trials have indicated that a mucosal
immune response to a crucial antigen(s) of mutans streptococci can influence the
pathogenesis of dental caries. Evidence that this antigen(s) is appropriate for use
in a vaccine against dental caries, as well as evidence for an appropriate target
population of individuals and a logical time of administration, has now emerged.
factor de impacto:
índice de citaciones del artículo: 20
numero de publicaciones: 81
Quartil: Q1
autor : Taubman, Martin A; Nash, David A
año: 2006
Construction of a new fusion anti-caries DNA vaccine. Construcción de una vacuna de ADN anti- caries nueva fusión
Abstract
Mutans streptococci (MS) are generally considered to be the principal etiological
agent of dental caries. MS have two important virulence factors: cell- surface protein
PAc and glucosyltransferases (GTFs). GTFs have two functional domains: an Nterminal
catalytic sucrose-binding domain (CAT) and a C-terminal glucan-binding
domain (GLU). A fusion anti-caries DNA vaccine, pGJA-P/VAX, encoding two
important antigenic domains, PAc and GLU, of S. mutans, was successful in
reducing the levels of dental caries caused by S. mutans in gnotobiotic animals.
However, its protective effect against S. sobrinus infection proved to be weak. Does
the DNA vaccine need an antigen of S. sobrinus to enhance its ability to inhibit
infection? To answer this question, in this study, we cloned the catalytic (cat)
fragment of S. sobrinus gtf-I, which demonstrated its ability to inhibit water-insoluble
glucan synthesis by S. sobrinus, into pGJA-P/VAX to produce a new anti-caries DNA
vaccine.
factor de impacto: 4.688
índice de citaciones del artículo: 19
número de publicaciones: 41
quartil: Q1
autor :Niu, Yongsheng
Current status of a mucosal vaccine against dental caries
Abstract
The evidence of a specific bacterial cause of dental caries and of the function of the
salivary glands as an effector site of the mucosal immune system has provided a
scientific basis for the development of a vaccine against this highly prevalent and
costly oral disease. Research efforts towards developing an effective and safe caries
vaccine have been facilitated by progress in molecular biology, with the cloning and
functional characterization of virulence factors from mutans streptococci, the
principal causative agent of dental caries, and advancements in mucosal
immunology, including the development of sophisticated antigen delivery systems
and adjuvants that stimulate the induction of salivary immunoglobulin A antibody
responses. Cell-surface fibrillar proteins, which mediate adherence to the salivary
pellicle, and glucosyltransferase enzymes, which synthesize adhesive glucans and
allow microbial accumulation, are virulence components of mutans streptococci, and
primary candidates for a human caries vaccine. Infants, representing the primary
target population for a caries vaccine, become mucosally immunocompetent and
secrete salivary immunoglobulin A antibodies during the first weeks after birth,
whereas mutans streptococci colonize the tooth surfaces at a discrete time period
that extends around 26 months of life. Therefore, immunization when infants are
about one year old may establish effective immunity against an ensuing colonization
attempts by mutans streptococci. The present review critically evaluates recent
progress in this field of dental research and attempts to stress the protective potential
as well as limitations of caries immunization.
Revista: molecular oral microbiology
Factor de impacto: 2.784
Scimago: Q1
Autor: G. Hajishengallis and S. M. Michalek
publicaciones del autor:135
Citaciones del artículo: 110
Año:1999
